Journal: Cell host & microbe

Article Title: Defined human Clostridia consortia reverse colitis via dual effects of tryptophan metabolites on microbiota and immunity

doi: 10.1016/j.chom.2026.08.003

Figure Lengend Snippet:

Article Snippet: Mouse Naïve CD4+ T Cell Isolation Kit , Miltenyi Biotec , Cat# 130–104-453.

Techniques: Blocking Assay, Control, Isolation, Variant Assay, Recombinant, Yeast, Starch, Electron Microscopy, Bacteria, Enzyme-linked Immunosorbent Assay, DNA Extraction, cDNA Synthesis, Cell Isolation, Staining, Sample Prep, Software, Sequencing, Qubit, Mass Spectrometry

Journal: Cell host & microbe

Article Title: Defined human Clostridia consortia reverse colitis via dual effects of tryptophan metabolites on microbiota and immunity

doi: 10.1016/j.chom.2026.08.003

Figure Lengend Snippet: (A and B) Germ-free (GF) Il10 -eGFP reporter mice were colonized with vehicle (Eggerth-Gagnon media), the 11- or 17-mix Clostridium strain consortia (2 × 10 7 CFU in 200 μL) for 2 weeks. cLP GFP (IL-10) + , FOXP3 + CD4 + T, and FOXP3 neg CD4 + T cells were analyzed by flow cytometry. N = 5−7/group. (B) Representative plots. (C) cLP RORγt+FOXP3+CD4+ T cells in GF WT mice 2 weeks after vehicle, 11-mix, or 17-mix. N = 4−5/group. (D) Cecal luminal metabolites from GF Il10 -eGFP reporter mice treated with vehicle, 11-mix, or 17-mix were analyzed by GC/MS and LC-MS/MS. Heat map shows log2-transformed, Z-scored metabolite abundance with FDR-corrected ANOVA q < 0.05 and Tukey post-hoc comparisons. Colored boxes indicate p < 0.05 red (Dn: decreased); green (Up: increased) boxes or p ≥ 0.05 white denotes no significant change (N.S.) compared with vehicle. N = 5−7/group. Arrows indicate AhR agonists/activators. (E) Selected metabolites. Data are mean ± SE; each point represents one mouse. One-way ANOVA with Tukey test, *p < 0.05, ***p < 0.001, ****p < 0.0001.

Article Snippet: Mouse Naïve CD4+ T Cell Isolation Kit , Miltenyi Biotec , Cat# 130–104-453.

Techniques: Flow Cytometry, Gas Chromatography-Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Transformation Assay

Journal: Cell host & microbe

Article Title: Defined human Clostridia consortia reverse colitis via dual effects of tryptophan metabolites on microbiota and immunity

doi: 10.1016/j.chom.2026.08.003

Figure Lengend Snippet: (A) Germ-free Rag2 −/− mice were colonized with donor-A human stool, received naïve CD4 + T cells 2 weeks later, and were treated with vehicle, 11-mix, or 17-mix (2 × 10 7 CFU in 200 μL) beginning 4 weeks after transfer. N = 7−8/group. (B) Stool images and scores. (C) Serial f-Lcn. The y-axis break retains an outlier while showing lower-range data. (D) Representative photomicrographs and histological score of 3 combined segments (cecum, proximal colon (Pro-colon), and rectum). Yellow arrowheads indicate infiltrating cells. Bar = 100 μm. (E) Colonic mRNA by qPCR relative to vehicle. N = 4/group. (F) Significant family- and genus-level stool microbiota changes by 16S rRNA sequencing. N = 4/group. Data are mean ± SE; each point represents one mouse. One-way ANOVA with Tukey test for B, D-F; Kruskal-Wallis with Dunn’s test for C. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Mouse Naïve CD4+ T Cell Isolation Kit , Miltenyi Biotec , Cat# 130–104-453.

Techniques: Adoptive Transfer Assay, Sequencing

Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

Article Snippet: Note: If no FACS device is available, the sorting of naïve T cells can be performed using a naïve MACS Sort Kit from Miltenyi Biotec (130-104-453 ).

Techniques: Cell Culture, Dot Blot, Luminex